Buaas, F. W., Gardiner, J. R., Clayton, S., Val, P., Swain, A. (2012) In vivo evidence for the crucial role of SF1 in steroid-producing cells of the testis, ovary and adrenal gland. DEVELOPMENT, 139 (24). pp. 4561-4570. ISSN 0950-1991

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Abstract

Adrenal and gonadal steroids are essential for life and reproduction. The orphan nuclear receptor SF1 (NR5A1) has been shown to regulate the expression of enzymes involved in steroid production in vitro. However, the in vivo role of this transcription factor in steroidogenesis has not been elucidated. In this study, we have generated steroidogenic-specific Cre-expressing mice to lineage mark and delete Sf1 in differentiated steroid-producing cells of the testis, the ovary and the adrenal gland. Our data show that SF1 is a regulator of the expression of steroidogenic genes in all three organs. In addition, Sf1 deletion leads to a radical change in cell morphology and loss of identity. Surprisingly, sexual development and reproduction in mutant animals were not compromised owing, in part, to the presence of a small proportion of SF1-positive cells. In contrast to the testis and ovary, the mutant adult adrenal gland showed a lack of Sf1-deleted cells and our studies suggest that steroidogenic adrenal cells during foetal stages require Sf1 to give rise to the adult adrenal population. This study is the first to show the in vivo requirements of SF1 in steroidogenesis and provides novel data on the cellular consequences of the loss of this protein specifically within steroid-producing cells.

Item Type:	Article
Authors (ICR Faculty only):	Swain, Amanda
All Authors:	Buaas, F. W., Gardiner, J. R., Clayton, S., Val, P., Swain, A.
Additional Information:	ISI Document Delivery No.: 041PO Times Cited: 0 Cited Reference Count: 41 Buaas, F. William Gardiner, Jennifer R. Clayton, Sally Val, Pierre Swain, Amanda Biotechnology and Biological Sciences Research Council (BBSRC) [BB/C517825/1]; Medical Research Council (MRC) [G0901645] This work was supported by the Biotechnology and Biological Sciences Research Council (BBSRC) [BB/C517825/1 to F. W. B.]; and the Medical Research Council (MRC) [G0901645 to J.R.G.]. Deposited in PMC for release after 6 months. Company of biologists ltd Cambridge
Uncontrolled Keywords:	Leydig cell differentiation Adrenal development Gonad development Steroidogenic factor 1 Steroidogenic gene regulation Mouse ventromedial hypothalamic nucleus leydig-cells gene-expression factor-1 differentiation receptor gonad identification transcription fetal
Research teams:	ICR divisions > Cancer Biology > Development and Cancer
Depositing User:	Barry Jenkins
Date Deposited:	02 Jan 2013 16:13
Last Modified:	02 Jan 2013 16:13
URI:	http://publications.icr.ac.uk/id/eprint/12141

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