Chung, Y. L., El Akabawy, G., So, P. W., Solanky, B. S., Leach, M. O., Modo, M. (2013) Profiling metabolite changes in the neuronal differentiation of human striatal neural stem cells using H-1-magnetic resonance spectroscopy. NEUROREPORT, 24 (18). pp. 1035-1040. ISSN 0959-4965

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Abstract

Objective Neural stem cells (NSCs) have been found to play an increasing clinical role in stroke. However, at present, it is not yet possible to noninvasively monitor their differentiation once implanted into the brain. Methods Here, we describe the use of high-resolution 1 H-magnetic resonance spectroscopy (MRS) to define a metabolite profile of undifferentiated human striatal NSCs from the STROC05 cell line and their differentiation after 3-weeks of treatment with purmorphamine. Results The undifferentiated conditions were characterized by similar to 95% of cells expressing nestin and similar to 77% being Ki67(+)ve, indicating that these were still proliferating. Phosphophocholine + glycerophosphocholine (PC+GPC) as well as myo-Inositol (mI) were increased in these cells. PC+GPC and mI were markedly reduced upon differentiation, potentially serving as markers of the NSC state. Upon differentiation (similar to 45% neurons, similar to 30% astrocytes, similar to 13% oligodendrocytes), the concentration of many metabolites decreased in absolute value. The decreasing trend of the N-acetyl-aspartate level was observed in differentiated cells when compared with NSCs. An increase in plasmalogen (enriched in myelin sheets) could potentially serve as a marker of oligodendrocytes. Conclusion These metabolite characteristics of undifferentiated and differentiated NSCs provide a basis for exploration of their possible use as markers of differentiation after cell transplantation. (C) 2013 Wolters Kluwer Health | Lippincott Williams & Wilkins.

Item Type:	Article
Authors (ICR Faculty only):	Chung, Yuen-Li and Leach, Martin
All Authors:	Chung, Y. L., El Akabawy, G., So, P. W., Solanky, B. S., Leach, M. O., Modo, M.
Additional Information:	ISI Document Delivery No.: 299DO Times Cited: 0 Cited Reference Count: 26 Chung, Yuen-Li El Akabawy, Gehan So, Po-Wah Solanky, Bhavana S. Leach, Martin O. Modo, Michel MRC [G0802552]; EU [201842-ENCITE]; RCUK; Egyptian Government [MM45/07]; CR-UK; EPSRC Cancer Imaging Centre; MRC; Department of Health (UK) [C1060/A10334]; NHS This work was supported by an MRC (G0802552) and an EU Framework VII Grant (201842-ENCITE). M. M. was the recipient of an RCUK fellowship. G. E.-A. was the recipient of a PhD studentship from the Egyptian Government (MM45/07). Y.-L.C. and M.O.L. acknowledge the support received from the CR-UK and EPSRC Cancer Imaging Centre in association with the MRC and Department of Health (UK) Grant C1060/A10334, NHS funding to the NIHR Biomedical Research Centre. Lippincott williams & wilkins Philadelphia
Uncontrolled Keywords:	DARPP-32 ganglionic eminence H-1-magnetic resonance spectroscopy human metabolites neural stem cell neuronal differentiation purmorphamine striatum human brain in-vivo nmr-spectroscopy h-1-nmr astrocytes expression stroke tissue
Research teams:	ICR divisions > Radiotherapy and Imaging > Magnetic Resonance
Depositing User:	Users 11 not found.
Date Deposited:	27 Feb 2014 13:03
Last Modified:	27 Feb 2014 13:03
URI:	http://publications.icr.ac.uk/id/eprint/13044

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