Yuan, Y. Y. (2015) Modelling the spatial heterogeneity and molecular correlates of lymphocytic infiltration in triple-negative breast cancer. JOURNAL OF THE ROYAL SOCIETY INTERFACE, 12 (103). ISSN 1742-5689

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Abstract

Lymphocytic infiltration is associated with a favourable prognosis and predicts response to chemotherapy in many cancer types, including the aggressive triple-negative breast cancer (TNBC). However, it is not well understood owing to the high levels of spatial heterogeneity within tumours, which is difficult to analyse by traditional pathological assessment. This paper describes an unbiased methodology to statistically model the spatial distribution of lymphocytes among tumour cells based on automated analysis of haematoxylin-and-eosin-stained whole-tumour section images, which is applied to two independent TNBC cohorts of 181 patients with matched microarray gene expression data. The novelty of the proposed methodology is the fusion of image analysis and statistical modelling for an integrative understanding of intratumour heterogeneity of lymphocytic infiltration. Using this methodology, a quantitative measure of intratumour lymphocyte ratio is developed and found to be significantly associated with disease-specific survival in both TNBC cohorts independent to standard clinical parameters. The proposed image-based measure compares favourably to a number of gene expression signatures of immune infiltration. In addition, heterogeneous immune infiltration at the morphological level is reflected at the molecular scale and correlated with increased expression of CTLA4, the target of ipilimumab. Taken together, these results support the fusion of high-throughput image analysis and statistical modelling to offer reproducible and robust biomarkers for the objective identification of patients with poor prognosis and treatment options.

Item Type:	Article
Authors (ICR Faculty only):	Yuan, Yinyin
All Authors:	Yuan, Y. Y.
Additional Information:	ISI Document Delivery No.: AW3BE Times Cited: 0 Cited Reference Count: 39 Yuan, Yinyin Royal soc London
Uncontrolled Keywords:	image analysis immune response tumour microenvironment statistical modelling tumors chemotherapy expression apobec3g predicts survival ctla-4 cells
Research teams:	ICR divisions > Molecular Pathology > Computational Pathology & Integrated Genomics
Depositing User:	Alexander Smithson
Date Deposited:	16 Jan 2015 15:30
Last Modified:	16 Jan 2015 15:30
URI:	http://publications.icr.ac.uk/id/eprint/13821

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