Bakal, C., Linding, R., Llense, F., Heffern, E., Martin-Blanco, E., Pawson, T., Perrimon, N. (2008) Phosphorylation networks regulating JNK activity in diverse genetic backgrounds. SCIENCE, 322 (5900). pp. 453-456. ISSN 0036-8075

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Abstract

Cellular signaling networks have evolved to enable swift and accurate responses, even in the face of genetic or environmental perturbation. Thus, genetic screens may not identify all the genes that regulate different biological processes. Moreover, although classical screening approaches have succeeded in providing parts lists of the essential components of signaling networks, they typically do not provide much insight into the hierarchical and functional relations that exist among these components. We describe a high-throughput screen in which we used RNA interference to systematically inhibit two genes simultaneously in 17,724 combinations to identify regulators of Drosophila JUN NH2-terminal kinase (JNK). Using both genetic and phosphoproteomics data, we then implemented an integrative network algorithm to construct a JNK phosphorylation network, which provides structural and mechanistic insights into the systems architecture of JNK signaling.

Item Type:	Article
Authors (ICR Faculty only):	Linding, Rune
All Authors:	Bakal, C., Linding, R., Llense, F., Heffern, E., Martin-Blanco, E., Pawson, T., Perrimon, N.
Uncontrolled Keywords:	RECEPTOR TYROSINE KINASE; CELL POLARITY; DORSAL CLOSURE; DROSOPHILA; PATHWAY; PHOSPHATASE; MOVEMENT; INVASION; ENCODES; PROTEIN
Research teams:	Closed research groups > Cellular and Molecular Logic
Depositing User:	Users 10 not found.
Date Deposited:	11 Nov 2008 10:35
Last Modified:	21 Jan 2014 15:56
URI:	http://publications.icr.ac.uk/id/eprint/7140

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