Drake, K. M., Ruteshouser, E. C., Natrajan, R., Harbor, P., Wegert, J., Gessler, M., Pritchard-Jones, K., Grundy, P., Dome, J., Huff, V., Jones, C., Aldred, M. A. (2009) Loss of Heterozygosity at 2q37 in Sporadic Wilms' Tumor: Putative Role for miR-562. CLINICAL CANCER RESEARCH, 15 (19). pp. 5985-5992. ISSN 1078-0432

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Abstract

Purpose: Wilms' tumor is a childhood cancer of the kidney with an incidence of similar to 1 in 10,000. Cooccurrence of Wilms' tumor with 2q37 deletion syndrome, an uncommon constitutional chromosome abnormality, has been reported previously in three children. Given these are independently rare clinical entities, we hypothesized that 2q37 harbors a tumor suppressor gene important in Wilms' tumor pathogenesis. Experimental Design: To test this, we performed loss of heterozygosity analysis in a panel of 226 sporadic Wilms' tumor samples and mutation analysis of candidate genes. Results: Loss of heterozygosity was present in at least 4% of cases. Two tumors harbored homozygous deletions at 2q37.1, supporting the presence of a tumor suppressor gene that follows a classic two-hit model. However, no other evidence of second mutations was found, suggesting that heterozygous deletion alone may be sufficient to promote tumorigenesis in concert with other genomic abnormalities. We show that miR-562, a microRNA within the candidate region, is expressed only in kidney and colon and regulates EYA1, a critical gene for renal development. miR-562 expression is reduced in Wilms' tumor and may contribute to tumorigenesis by deregulating EYA1. Two other candidate regions were localized at 2q37.3 and 2qter, but available data from patients with constitutional deletions suggest that these probably do not confer a high risk for Wilms' tumor. Conclusions: Our data support the presence of a tumor suppressor gene at 2q37.1 and suggest that, in individuals with constitutional 2q37 deletions, any increased risk for developing Wilms' tumor likely correlates with deletions encompassing 2q37.1. (Clin Cancer Res 2009;15(19):5985-92)

Item Type:	Article
Authors (ICR Faculty only):	Pritchard-Jones, Kathy and Jones, Chris
All Authors:	Drake, K. M., Ruteshouser, E. C., Natrajan, R., Harbor, P., Wegert, J., Gessler, M., Pritchard-Jones, K., Grundy, P., Dome, J., Huff, V., Jones, C., Aldred, M. A.
Uncontrolled Keywords:	ALBRIGHT HEREDITARY OSTEODYSTROPHY; GERMLINE MUTATIONS; TERMINAL DELETION; GENE-EXPRESSION; SUPPRESSOR GENE; ARRAY CGH; MICRORNAS; PHENOTYPE; WT1; ABNORMALITIES
Funding Acknowledgement:	National Wilms' Tumor Study Group ; Children's Oncology Group ; Cooperative Human Tissue Network ; National Cancer Institute
Funding Text:	We thank the National Wilms' Tumor Study Group, the Children's Oncology Group, and the Cooperative Human Tissue Network, funded by the National Cancer Institute, for access to samples; the Genomics and Integrative Genomic Analysis Cores at the Lerner Research Institute for genotyping services and bioinformatics support; and Dr. Bryan Williams for kindly providing the WiT-49 cells.
Research teams:	Closed research groups > Childhood Cancer Biology ICR divisions > Cancer Therapeutics > Paediatric Molecular Pathology ICR divisions > Molecular Pathology > Paediatric Molecular Pathology
Depositing User:	Users 10 not found.
Date Deposited:	19 Oct 2009 10:30
Last Modified:	10 Feb 2010 11:52
URI:	http://publications.icr.ac.uk/id/eprint/8830

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